严志祥+李啸峰+燕茹:多组学揭示COVID-19患者肠道内分子变化
  • 纳入新冠患者13例,健康对照21人,在新冠肺炎症状出现到94天对9个纵向时间点进行粪便采样和多组学分析;
  • 中性粒细胞脱粒和迁移相关蛋白的表达及糖基化被抑制,而蛋白酶的表达和糖化则上调;
  • Ig重链可变域下调,IgA重链恒定域、IgGFc结合蛋白和J链的糖化被聚糖特异性改变抑制;
  • 肠道有益菌减少和有害代谢物增加,Ig重链可变域的减少与某些拟杆菌增加有关;
  • 肠道神经酰胺脂下调,大多数肠道表型在症状发作两个月后仍未恢复。
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章台柳
中山大学严志祥、李啸峰和澳门大学燕茹与团队在Analytica Chimica Acta发表文章,通过对新冠患者的粪便样本进行纵向动态性多组学分析,揭示出肠道分子结构被广泛干扰,或在COVID-19的症状发展中起重要作用。同时研究结果指出,大多数肠道表型在症状发作两个月后仍未恢复,需要对COVID-19恢复患者的肠道分子和菌群变化进行长期的持续性研究。
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Fecal multi-omics analysis reveals diverse molecular alterations of gut ecosystem in COVID-19 patients

粪便多组学揭示新冠肺炎患者肠道生态系统的不同分子改变

10.1016/j.aca.2021.338881

2021-07-26, Article

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Gut ecosystem has profound effects on host physiology and health. Gastrointestinal (GI) symptoms were frequently observed in patients with COVID-19. Compared with other organs, gut antiviral response can result in more complicated immune responses because of the interactions between the gut microbiota and host immunity. However, there are still large knowledge gaps in the impact of COVID-19 on gut molecular profiles and commensal microbiome, hindering our comprehensive understanding of the pathogenesis of SARS-CoV-2 and the treatment of COVID-19. We performed longitudinal stool multi-omics profiling to systemically investigate the molecular phenomics alterations of gut ecosystem in COVID-19. Gut proteomes of COVID-19 were characterized by disturbed immune, proteolysis and redox homeostasis. The expression and glycosylation of proteins involved in neutrophil degranulation and migration were suppressed, while those of proteases were upregulated. The variable domains of Ig heavy chains were downregulated and the overall glycosylation of IgA heavy chain constant regions, IgGFc-binding protein, and J chain were suppressed with glycan-specific variations. There was a reduction of beneficial gut bacteria and an enrichment of bacteria derived deleterious metabolites potentially associated with multiple types of diseases (such as ethyl glucuronide). The reduction of Ig heave chain variable domains may contribute to the increase of some Bacteroidetes species. Many bacteria ceramide lipids with a C17-sphingoid based were downregulated in COVID-19. In many cases, the gut phenome did not restore two months after symptom onset. Our study indicates widely disturbed gut molecular profiles which may play a role in the development of symptoms in COVID-19. Our findings also emphasis the need for ongoing investigation of the long-term gut molecular and microbial alterations during COVID-19 recovery process. Considering the gut ecosystem as a potential target could offer a valuable approach in managing the disease.

First Authors:
Feixiang He

Correspondence Authors:
Ru Yan,Xiaofeng Li,Zhixiang Yan

All Authors:
Feixiang He,Ting Zhang,Kewen Xue,Zhaoxiong Fang,Guanmin Jiang,Siwen Huang,Kexue Li,Zhiqiang Gu,Honggang Shi,Zhenyi Zhang,Huijin Zhu,Lu Lin,Jialin Li,Fei Xiao,Hong Shan,Ru Yan,Xiaofeng Li,Zhixiang Yan

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