• 分析肝硬化患者的肠道菌群代谢组;
  • 选取肝功能分级为A及B的患者各5名,以及3名健康人,收集粪便菌群进行分析;
  • 相比于健康人,菌群-代谢产物间的相关性在肝硬化患者中发生变化;
  • 肝硬化患者的粪便菌群丰度降低,拟杆菌门丰度降低,厚壁菌门及变形菌门丰度增加;
  • 在肝硬化患者的肠道菌群代谢组中,胺、不饱和脂肪酸、短链脂肪酸浓度较高,糖醇、氨基酸浓度较低。

Community-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B Patients



2016-11-16, Article

Abstract & Authors:展开

The gut flora are widely involved in the cometabolism with the host and have evident effects on the metabolic phenotype of host. This study performed a metabolome analysis of the intestinal microbiota specific for liver cirrhosis. The study population included patients with Child-Turcotte-Pugh score of A (AP, n = 5) and B (BP, n = 5), and control subjects (NM, n = 3). Metagenomic DNA from fecal microbiota was extracted followed by metagenomic sequencing through Illumina MiSeq high throughput sequencing of 16S rRNA regions. The detection of metabolites from fecal samples was performed using high-performance liquid phase chromatography and gas chromatography coupled with tandem mass spectrometry. Intestinal microbiota community and metabolite analysis both showed separation of cirrhotic patients from control participants, moreover, the microbiota–metabolite correlations changed in cirrhotic patients. Fecal microbiota from cirrhotic patients, with the reduced diversity, contained a decreased abundance of Bacteroidetes and an increased abundance of Firmicutes and Proteobacteria compared with the normal samples. Analysis of metabolome revealed a remarkable change in the metabolic potential of the microbiota in cirrhotic patients, with specific higher concentrations of amine, unsaturated fatty acid, and short-chain fatty acids, and lower concentrations of sugar alcohol and amino acid, suggesting the initial equilibrium of gut microbiota community and co-metabolism with the host were perturbed by cirrhosis. Our study illustrated the relationship between fecal microbiota composition and metabolome in cirrhotic patients, which may improve the clinical prognosis of cirrhosis.

First Authors:
Xiao Wei

Correspondence Authors:
Jing Yuan,Yansong Sun

All Authors:
Xiao Wei,Shan Jiang,Xiangna Zhao,Huan-Huan Liu,Weishi Lin,Boxing Li,Jing Lu,Yansong Sun,Jing Yuan