陈峰+陈宁:基因型、饮食、年龄等对小鼠肠道菌群的塑造作用
  • 纳入Rbp4-/-小鼠(遗传性维生素A缺陷小鼠)、Rbp4+/-小鼠和野生型小鼠,在4或7周龄开始28天的低维生素A饮食干预;
  • Rbp4缺陷影响肠道菌群组成,Akk菌减少、拟杆菌属增多,脱硫弧菌属、Barnesiella、梭菌属XlVa、乳杆菌属等发生变化;
  • 肠道菌群对饮食干预快速响应并逐渐改变,饮食干预起始越早,菌群失调程度越高;
  • 年龄对菌群的影响较弱但持久;
  • 饮食和年龄对肠道菌群的塑造作用因基因型而异,提示基因型可能对菌群塑造起主导作用。
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mildbreeze
北京大学口腔医院陈峰团队和北京大学人民医院陈宁团队合作,在Journal of Genetics and Genomics发表研究,以遗传性维生素A缺陷小鼠为模型,发现宿主基因型、饮食、年龄和饮食干预起始年龄等因素,在不同程度上影响了肠道菌群的组成。
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The role of genotype and diet in shaping gut microbiome in a genetic Vitamin A deficient mouse model

在遗传性维生素A缺陷小鼠模型中,基因型和饮食在塑造肠道菌群中的作用

10.1016/j.jgg.2021.08.015

2021-09-16, Article

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Multi-factors have been reported to affect the gut microbiome, including genotype, age, diet, and nutrition. However, few reports have investigated the relative capacity of different factors to shape the gut microbiome in a single study. Our design used a genetic Vitamin A deficient mouse model, the Rbp4−/− mouse, feeding with the low Vitamin A diets at different ages of initiation (4 or 7 weeks) for 28 days. Fecal samples were collected for bacterial profiling at seven time points after diet controlling. With RBP4 depletion, Akkermansia decreased and Bacteroides increased, while Desulfovibrio, Barnesiella, Clostridium_XlVa, and Lactobacillus fluctuated. The bacterial community swiftly adjusted with the Vitamin A-deficient diet administration and gradually changed (e.g., decrease of Barnesiella and increase of Desulfovibrio). Age exerted a relatively weaker but long-last influence. At an earlier age to feed a Vitamin-A deficient diet, a higher microbial dysbiosis index (MDI) will be valued. Of note, the shaping effects of diet and age on the bacterial community varied with the difference of genotype, which might indicate a greater role of genotype than diet and age in shaping the gut microbiome.

First Authors:
Jun Xu,Jieni Zhang

Correspondence Authors:
Ning Chen,Feng Chen

All Authors:
Jun Xu,Jieni Zhang,Bo-Hui Sun,Qing Liu,Juan Ma,Qian Zhang,Yong-Xin Liu,Ning Chen,Feng Chen

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