Abstract & Authors:展开
Objective: Many cancers engage embryonic genes for rapid growth and evading the immune system. SOX9 has been upregulated in many tumours, yet the role of SOX9 in mediating immunosuppressive tumour microenvironment is unclear. Here, we aim to dissect the role of SOX9-mediated cancer stemness attributes and immunosuppressive microenvironment in advanced gastric adenocarcinoma (GAC) for novel therapeutic discoveries.
Methods: Bulk RNAseq/scRNA-seq, patient-derived cells/models and extensive functional studies were used to identify the expression and functions of SOX9 and its target genes in vitro and in vivo. Immune responses were studied in PBMCs or CD45+ immune cells cocultured with tumour cells with SOX9high or knockout and the KP-Luc2 syngeneic models were used for efficacy of combinations.
Results : SOX9 is one of the most upregulated SOX genes in GAC and highly expressed in primary and metastatic tissues and associated with poor prognosis. Depletion of SOX9 in patient-derived GAC cells significantly decreased cancer stemness attributes, tumour formation and metastases and consistently increased CD8+ T cell responses when cocultured with PBMCs/CD45+ cells from GAC patients. RNA sequencing identified the leukaemia inhibitory factor (LIF) as the top secreted molecule regulated by SOX9 in tumour cells and was enriched in malignant ascites and mediated SOX9-induced M2 macrophage repolarisation and inhibited T cell function.
Conclusion: Epithelial SOX9 is critical in suppressing CD8+ T cell responses and modified macrophage function in GAC through the paracrine LIF factor. Cotargeting LIF/LIFR and CSF1R has great potential in targeting SOX9-mediated cancer stemness, T cell immunosuppression and metastases suggesting the novel combination therapy against advanced GAC.
Jaffer A Ajani,Shumei Song
Yibo Fan,Yuan Li,Xiaodan Yao,Jiangkang Jin,Ailing Scott,Bovey Liu,Shan Wang,Longfei Huo,Ying Wang,Ruiping Wang,Melissa Pool Pizzi,Lang Ma,Shan Shao,Matheus Sewastjanow-Silva,Rebecca Waters,Deyali Chatterjee,Bin Liu,Namita Shanbhag,Guang Peng,George Adrian Calin,Pawel Karol Mazur,Samir M Hanash,Jo Ishizawa,Yuki Hirata,Osamu Nagano,Zhenning Wang,Linghua Wang,Wa Xian,Frank McKeon,Jaffer A Ajani,Shumei Song