脐带血微生物组特征或是不良妊娠结局的标志物
创作:Epi汪 审核:Epi汪 2022年08月26日
  • 纳入60名>20周的死产胎儿、176名活产胎儿,采集脐带血进行菌群检测;
  • 在调整了潜在混杂因素之后,死产组中细菌的检出率和丰度都明显增加;
  • 随机森林模型通过脐带血菌群区分死产和活产的准确度为0.76,其中B组链球菌是死产胎儿中丰度最高的物种;
  • 而在早期早产胎儿脐带血中,一组口腔厌氧菌丰度增加,包括放线菌属、弯曲杆菌属、梭杆菌属、消化链球菌属、卟啉单胞菌属、普雷沃氏菌属等;
  • 脐带血微生物特征或许是不良妊娠结局的标志物。
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Epi汪
本研究通过对胎儿脐带血的微生物组学测序分析,发现B组链球菌或许与死胎的发生风险有关,而口腔致病菌的感染可能与早产的发生有关。本研究为探索不良妊娠结局的病因提供了新的思路。
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mBio [IF:7.786]

Microbial Analysis of Umbilical Cord Blood Reveals Novel Pathogens Associated with Stillbirth and Early Preterm Birth

脐带血微生物分析揭示了与死产和早产相关的新型病原体

10.1128/mbio.02036-22

2022-08-22, Article

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Stillbirths account for half of all perinatal mortality, but the underlying cause of a significant portion of the cases remains unknown. We set out to test the potential role and extent of microbial infection in stillbirth through a case-control analysis of fetal cord blood collected from the multisite Stillbirth Collaborative Research Network. Cases (n = 60) were defined as stillbirths at >20 weeks of gestation, and controls (n = 176) were live births. The bacterial presence, abundance, and composition were analyzed by endpoint PCR of full-length 16S rRNA and the V4 amplicon sequence variants (ASVs). The results demonstrate that bacterial prevalence and abundance were both significantly increased in stillbirth, even after adjusting for maternal age, race, body mass index, number of pregnancies, gestational age, and multiple gestations. Composition of bacterial communities in the cord blood also differed significantly. Using a group of 25 ASVs differentially abundant between the two groups, a Random Forest classification model achieved an accuracy score of 0.76 differentiating stillbirth and live birth, with Group B Streptococcus as the most enriched species in stillbirth. Positive PCR was also significantly associated with early preterm birth. A group of oral anaerobes, including Actinomyces, Campylobacter, Fusobacterium, Peptostreptococcus, Porphyromonas, and Prevotella, were enriched in live early preterm birth, suggesting possible oral origin of infection. Our ASV-based microbiome analysis revealed specific candidate pathogens associated with infections in stillbirth and early preterm birth. The cord blood microbial signatures may be markers of adverse pregnancy outcomes. Our study will help identify possible mechanism of infection and improve our ability to prevent stillbirth and early preterm birth. IMPORTANCE Stillbirth accounts for half of all perinatal mortality, but the underlying cause of a substantial portion of all cases remains elusive. We examined the umbilical cord blood microbiome in stillbirths (n = 60) and live births (n = 176) and discovered that the bacterial prevalence and abundance were significantly higher in stillbirths than live births. The microbial compositions also differed significantly. Group B Streptococcus was the most prevalent species detected in stillbirth. In addition, pathogens previously unknown to be associated with stillbirth were identified. A group of oral anaerobes including Fusobacterium nucleatum were found to be specifically enriched in the cord blood in early preterm live birth. This is by far the most comprehensive study to examine the microbial signatures in umbilical cord blood. Cord blood microbial signatures may be markers for adverse birth outcomes. Detection of key microbial signatures will help identify individuals at risk and develop effective preventative strategies.

First Authors:
Emilie L Vander Haar #,Guojun Wu #

Correspondence Authors:
Yiping W Han

All Authors:
Emilie L Vander Haar #,Guojun Wu #,Cynthia Gyamfi-Bannerman,Charlene Thomas,Ronald J Wapner,Uma M Reddy,Liping Zhao,Robert M Silver,Robert L Goldenberg,Yiping W Han

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