秦环龙等:鉴定具核梭杆菌亚种的新方法
创作:aluba 审核:aluba 2021年08月02日
  • 对比分析53种具核梭杆菌菌株的基因组进行,鉴定出特异性基因标志物,以区分具核梭杆菌的4个亚种;
  • 针对nucleatum亚种及animalis亚种,分别鉴定出6个及3个特异性的基因区域,针对vincenti,鉴定出2个特异性的基因间隔区,针对polymorphum亚种,鉴定出3个特异性的基因区域组合;
  • 设计引物扩增上述基因标志物的保守区域,在结直肠癌患者的58份粪便样本及100份新鲜冷冻的肿瘤组织中,发现animalis亚种最普遍,而nucleatum较少被检测到。
主编推荐语
aluba
具核梭杆菌具有4个亚种,可能促进结直肠癌的发生发展,但现有方法难以区分这4个亚种。上海市第十人民医院的秦环龙团队与蔚青团队在Microbial Biotechnology上发表的一项最新研究,鉴定出了14种特异性的基因标志物,可基于PCR检测,在结直肠癌患者的粪便样本及肿瘤样本中鉴别具核梭杆菌的4个亚种。
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A newly developed PCR-based method revealed distinct Fusobacterium nucleatum subspecies infection patterns in colorectal cancer

基于PCR的新方法揭示结直肠癌中的具核梭杆菌亚种感染模式

10.1111/1751-7915.13900

2021-07-26, Article

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Fusobacterium nucleatum, which has four subspecies (nucleatum, animalis, vincentii and polymorphum), plays an important role in promoting colorectal cancer (CRC). However, as there is no efficient method of differentiating these subspecies in the context of a rich gut microbiota, the compositions in CRC remain largely unknown. In this study, a PCR-based differentiation method enabling profiling of F. nucleatum infection in CRC at the subspecies level was developed. Based on the analysis of 53 F. nucleatum genomes, we identified genetic markers specific to each subspecies and designed primers for the conserved sequences of those markers. The PCR performance of the primers was tested with F. nucleatum and non-nucleatum Fusobacterium strains, and complete consistence with taxonomy was achieved. Additionally, no non-specific amplification occurred when using human DNA. The method was then applied to faecal (n = 58) and fresh-frozen tumour tissue (n = 100) samples from CRC patients, and wide heterogeneity in F. nucleatum subspecies compositions in the gut microbiota among CRC patients was observed. Single-subspecies colonization was common, whereas coexistence of four subspecies was rare. Subspecies animalis was most prevalent, while nucleatum was not frequently detected. The results of this study contribute to our understanding of the pathogenicity of F. nucleatum at the subspecies level and the method developed has potential for clinical and epidemiological use.

First Authors:
Dexi Bi,Yin Zhu

Correspondence Authors:
Qing Wei,Huanlong Qin

All Authors:
Dexi Bi,Yin Zhu,Yaohui Gao,Hao Li,Xingchen Zhu,Rong Wei,Ruting Xie,Qing Wei,Huanlong Qin

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