从双胞胎看肠道微生物组相似性动力学
创作:往、昔℡ 审核:好雨 05月12日
  • 研究108对双胞胎上(胃、唾液)、下(粪便)消化道(GI)菌群,评估基因型、分娩方式、饮食、共居和老化对同/异卵双胞胎微生物组的影响;
  • 发现上、下GI整体菌群结构明显不同;
  • 上GI菌群结构由幽门螺杆菌(Hp)状态决定,阳性率达55%,可脱离卵型仅依据Hp感染与否将双胞胎分开;
  • 下GI菌群相似性由共居和老化决定,不受分娩方式和基因型影响;
  • 随着年龄增长,双胞胎菌群相似性下降,共有的细菌类群与年龄成反比,而非共有的则相反。
主编推荐语
好雨
EBioMedicine最新发表的文章,对双胞胎消化道微生物组的时空变化进行了分析,提示环境对菌群的影响或大于遗传。
关键字
延伸阅读本研究的原文信息和链接出处,以及相关解读和评论文章。欢迎读者朋友们推荐!
图片
EBioMedicine [IF:11.205]

Gut microbial similarity in twins is driven by shared environment and aging

双胞胎肠道微生物的相似度由同居环境和老化驱动

10.1016/j.ebiom.2022.104011

04-28, Article

Abstract & Authors:展开

Abstract:收起
Background: Human gut microbiome composition is influenced by genetics, diet and environmental factors. We investigated the microbial composition in several gastrointestinal (GI) compartments to evaluate the impact of genetics, delivery mode, diet, household sharing and aging on microbial similarity in monozygotic and dizygotic twins.
Methods: Fecal, biopsy and saliva samples were obtained from total 108 twins. DNA and/or RNA was extracted and the region V1-V2 of the 16S rRNA gene was amplified and sequenced. Bray-Curtis similarity was used for further microbiome comparisons, Mann-Whitney test was applied to evaluate the significant differences between groups and Spearman test was applied to reveal potential correlations between data.
Findings: The global bacterial profiles were grouped into two clusters separating the upper and lower GI. The upper GI microbiome composition was strictly dependent on the Helicobacter pylori status. With a positivity rate of 55%, H. pylori completely colonized the stomach and separated infected twins from non-infected twins irrespective of zygosity status. Lower GI microbiome similarity between the twins was defined mainly by household-sharing and aging; whereas delivery mode and host genetics had no influence. There was a progredient decrease in the bacterial similarity with aging. Shared vs. non-shared phylotypes analysis showed that in both siblings the shared phylotypes progressively diminished with aging, while the non-shared phylotypes increased.
Interpretation: Our findings strongly highlight the aging and shared household as they key determinants in gut microbial similarity and drift in twins irrespective of their zygotic state.
Funding: This work was supported by the grant of the Research Council of Lithuania (Project no. APP-2/2016) and also partially supported by the funds of European Commission through the “European funds for regional development” (EFRE) as well as by the regional Ministry of Economy, Science and Digitalization as part of the “LiLife” Project as part of the “Autonomy in old Age” research group (Project ID: ZS/2018/11/95324).

First Authors:
Ramiro Vilchez-Vargas,Jurgita Skieceviciene

Correspondence Authors:
Juozas Kupcinskas,Alexander Link

All Authors:
Ramiro Vilchez-Vargas,Jurgita Skieceviciene,Konrad Lehr,Greta Varkalaite,Cosima Thon,Mindaugas Urba,Egidijus Morkūnas,Laimutis Kucinskas,Karolina Bauraite,Denny Schanze,Martin Zenker,Peter Malfertheiner,Juozas Kupcinskas,Alexander Link

评论