张逸超+秦环龙+何敏:首次描绘GHPA患者肠道菌群的种水平特征
创作:Akkk 审核:章台柳 01月28日
  • 纳入28名GHPA患者和67名健康对照,比较术前和术后6个月的空腹血糖、生长激素、IGF-1等指标,并对术前粪便标本测序;
  • GHPA菌群多样性显著降低,颤杆菌和肠杆菌相对丰度显著增加,沙门别样杆菌、内脏臭气杆菌显著富集,而对照组中布劳特氏菌和Romboutsia属占多数,肠道菌群或可成为GHPA的生物标志物;
  • GHPA氨基酸代谢途径显著富集,对照组碳水化合物代谢途径富集;
  • 肠杆菌属与基线IGF-1显著正相关,可能与GHPA中GH/IGF-1轴密切相关。
主编推荐语
章台柳
先前的研究表明,在生长激素分泌型垂体腺瘤(GHPA)患者中,属水平的肠道菌群发生了显著改变。然而,目前还没有研究描述GHPA中肠道菌群在种水平上的状态。复旦附属华山医院张逸超、何敏与同济大学第十人民医院秦环龙作为共同通讯作者在Microbiology spectrum发表文章,首次在种水平对GHPA患者和健康对照的肠道菌群进行分析,揭示了其肠道菌群的失调,或为理解GHPA的病理生理过程提供参考。
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Characteristics of Gut Microbiota in Patients with GH-Secreting Pituitary Adenoma

生长激素分泌型垂体腺瘤患者的肠道菌群特征

10.1128/spectrum.00425-21

01-12, Article

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Prior study has demonstrated that gut microbiota at the genus level is significantly altered in patients with growth hormone (GH)-secreting pituitary adenoma (GHPA). Yet, no studies exist describing the state of gut microbiota at species level in GHPA. We performed a study using 16S rRNA amplicon sequencing in a cohort of patients with GH-secreting pituitary adenoma (GHPA,  = 28) and healthy controls ( = 67). Among them, 9 patients and 10 healthy controls were randomly chosen and enrolled in metagenomics shotgun sequencing, generating 280,426,512 reads after aligning to NCBI GenBank DataBase to acquire taxa information at the species level. Weighted UniFrac analysis revealed that microbial diversity was notably decreased in patients with GHPA, consistent with a previous study. With 16S rRNA sequencing, after correction for false-discovery rate (FDR), rank-sum test at the genus level revealed that the relative abundance of and was remarkably increased in patients and and genera predominated in the controls, augmented by additional LEfSe (linear discriminant analysis effect size) analysis. As for further comparison at the species level with metagenomics sequencing, rank-sum test together with LEfSe analysis confirmed the enrichment of Alistipes shahii and Odoribacter splanchnicus in the patient group. Notably, LEfSe analysis with metagenomics also demonstrated that sp. and sp. , derived from , were both significantly enriched in patients. Functional analysis showed that amino acid metabolism pathway was remarkably enriched in GHPA, while carbohydrate metabolism pathway was notably enriched in controls. Further, significant positive correlations were observed between and baseline insulin-like growth factor 1 (IGF-1), indicating that may be strongly associated with GH/IGF-1 axis in GHPA. Our data extend our insight into the GHPA microbiome, which may shed further light on GHPA pathogenesis and facilitate the exploration of novel therapeutic targets based on microbiota manipulation. Dysbiosis of gut microbiota is associated not only with intestinal disorders but also with numerous extraintestinal diseases. Growth hormone-secreting pituitary adenoma (GHPA) is an insidious disease with persistent hypersecretion of GH and IGF-1, causing increased morbidity and mortality. Researches have reported that the GH/IGF-1 axis exerts its own influence on the intestinal microflora. Here, the results showed that compared with healthy controls, GHPA patients not only decreased the alpha diversity of the intestinal flora but also significantly changed their beta diversity. Further, metagenomics shotgun sequencing in the present study exhibited that sp. and sp. were enriched in patients. Also, we were pleasantly surprised to find that the genus was strongly positively correlated with baseline IGF-1 levels. Collectively, our work provides the first glimpse of the dysbiosis of the gut microbiota at species level, providing a better understanding of the pathophysiological process of GHPA.

First Authors:
Ben Lin,Meng Wang,Renyuan Gao,Zhao Ye

Correspondence Authors:
Min He,Huanlong Qin,Yichao Zhang

All Authors:
Ben Lin,Meng Wang,Renyuan Gao,Zhao Ye,Yifei Yu,Wenqiang He,Nidan Qiao,Zengyi Ma,Chenxing Ji,Chengzhang Shi,Xiang Zhou,Yi Wang,Fangfang Zeng,Li Zhang,Wei Gong,Zhan Cao,Peng Zhou,Vladimir Melnikov,Hongying Ye,Yiming Li,Zhaoyun Zhang,Min He,Huanlong Qin,Yichao Zhang

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