浙大韩新燕等:粪菌移植增强菌群色氨酸代谢,改善肠道屏障
创作:女巫 审核:mildbreeze 2018年08月01日
  • 新生仔猪分为实验和对照组,前者一出生即接受来自健康成年猪的粪菌移植(FMT);
  • FMT调节仔猪的结肠菌群多样性和组成,并降低对脂多糖(LPS)诱导的上皮完整性破坏和严重炎症反应的易感性;
  • 受体仔猪的肠道代谢组功能改变,结肠腔中菌群衍生的色氨酸分解代谢物吲哚-3-乙酸显著增加,伴随细胞因子IL-22的上调和芳香烃受体的活化增强;
  • FMT对受体结肠中菌群色氨酸代谢的调节作用,可能在维持肠屏障中有潜在作用。
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mildbreeze
来自浙江大学韩新燕团队主导的研究,近期在Journal of Crohn's and Colitis发表。该研究使用仔猪模型,表明粪菌移植可通过调节菌群的色氨酸代谢,改善肠道屏障功能。
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Fecal Microbiota Transplantation Reduces Susceptibility to Epithelial Injury and Modulates Tryptophan Metabolism of Microbial Community in a Piglet Model

在仔猪模型中粪菌移植减少了对上皮损伤的易感性并调节了菌群的色氨酸代谢

10.1093/ecco-jcc/jjy103

2018-07-14, Article

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Background and Aims: Fecal microbiota transplantation [FMT] has shown promise as a treatment for inflammatory bowel disease [IBD]. Using a piglet model, our previous study indicated that exogenous fecal microbiota can enhance gastrointestinal health through enhancing intestinal barrier. However, specific connections between FMT-induced microbial changes and modulation of intestinal barrier still remain to be fully illustrated. Here, we aimed to determine the potential role of metabolic function of gut microbiota in the beneficial effects of FMT.
Methods: The influence of FMT on the maintenance of intestinal homeostasis was assessed by early-life gut microbiota intervention on newborn piglets and subsequent lipopolysaccharide [LPS] challenge. Analysis of the gut microbiome and metabolome was carried out by 16S rRNA sequencing and multiple mass spectrometry platforms.
Results: FMT modulated the diversity and composition of colonic microbiota and reduced the susceptibility to LPS-induced destruction of epithelial integrity and severe inflammatory response. Metabolomic analysis revealed functional changes of the gut metabolome along with significant increase of typical microbiota-derived tryptophan catabolite indole-3-acetic acid in the colonic lumen. Further, metagenomics prediction analysis based on 16S rRNA sequencing also demonstrated that FMT modulated the metabolic functions of gut microbiota associated with tryptophan metabolism and intestinal homeostasis, which coincided with up-regulation of cytokine interleukin-22 and enhanced activation of aryl hydrocarbon receptor in the recipient colon.
Conclusions: Our data reveal a regulatory effect of FMT on tryptophan metabolism of gut microbiota in the recipient colon, which may play a potential role in the maintenance of intestinal barrier.

First Authors:
Shijie Geng

Correspondence Authors:
Xinyan Han

All Authors:
Shijie Geng,Saisai Cheng,Yuan Li,Zhengshun Wen,Xin Ma,Xuemei Jiang,Yizhen Wang,Xinyan Han

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