创作:aluba 审核:aluba 2017年04月12日
  • BL6小鼠与Sv129小鼠对高脂饮食诱导的肥胖敏感性不同,抑制环氧酶活性可抑制前者而非后者的肥胖;
  • 无论是喂食高脂或低脂饮食,或是添加环氧酶抑制剂与否,两种小鼠的肠道菌群在分类及功能上均有差异;
  • 可产生丁酸盐的细菌在Sv129小鼠中丰度更高,与丙酸盐代谢相关的基因在BL6小鼠中丰度更高;
  • 结论:高脂饮食,而非肥胖,导致了肠道菌群的改变;
  • 两种小鼠肥胖易感性的差别部分是由于肠道中可产生丁酸盐及丙酸盐的细菌的丰度差异造成的。
Microbiome [IF:14.65]

High-fat feeding rather than obesity drives taxonomical and functional changes in the gut microbiota in mice



2017-04-08, Article

Abstract & Authors:展开

BACKGROUND: It is well known that the microbiota of high-fat (HF) diet-induced obese mice differs from that of lean mice, but to what extent, this difference reflects the obese state or the diet is unclear. To dissociate changes in the gut microbiota associated with high HF feeding from those associated with obesity, we took advantage of the different susceptibility of C57BL/6JBomTac (BL6) and 129S6/SvEvTac (Sv129) mice to diet-induced obesity and of their different responses to inhibition of cyclooxygenase (COX) activity, where inhibition of COX activity in BL6 mice prevents HF diet-induced obesity, but in Sv129 mice accentuates obesity.
RESULTS: Using HiSeq-based whole genome sequencing, we identified taxonomic and functional differences in the gut microbiota of the two mouse strains fed regular low-fat or HF diets with or without supplementation with the COX-inhibitor, indomethacin. HF feeding rather than obesity development led to distinct changes in the gut microbiota. We observed a robust increase in alpha diversity, gene count, abundance of genera known to be butyrate producers, and abundance of genes involved in butyrate production in Sv129 mice compared to BL6 mice fed either a LF or a HF diet. Conversely, the abundance of genes involved in propionate metabolism, associated with increased energy harvest, was higher in BL6 mice than Sv129 mice.
CONCLUSIONS: The changes in the composition of the gut microbiota were predominantly driven by high-fat feeding rather than reflecting the obese state of the mice. Differences in the abundance of butyrate and propionate producing bacteria in the gut may at least in part contribute to the observed differences in obesity propensity in Sv129 and BL6 mice.

First Authors:
Liang Xiao,Si Brask Sonne,Qiang Feng

Correspondence Authors:
Karsten Kristiansen

All Authors:
Liang Xiao,Si Brask Sonne,Qiang Feng,Ning Chen,Zhongkui Xia,Xiaoping Li,Zhiwei Fang,Dongya Zhang,Even Fjære,Lisa Kolden Midtbø,Muriel Derrien,Floor Hugenholtz,Longqing Tang,Junhua Li,Jianfeng Zhang,Chuan Liu,Qin Hao,Ulla Birgitte Vogel,Alicja Mortensen,Michiel Kleerebezem,Tine Rask Licht,Huanming Yang,Jian Wang,Yingrui Li,Manimozhiyan Arumugam,Jun Wang,Lise Madsen,Karsten Kristiansen