郑大一附院任志刚/余祖江+李兰娟院士:新冠如何影响口腔和肠道菌群及脂质组?
  • 收集中国中部和东部的392份舌苔、172份粪便和155份血清样本;
  • 与健康对照(HC)相比,确诊病人(CP)的口腔和粪便微生物群的多样性显著降低,口腔中丁酸产生菌减少,脂多糖产生菌增多;
  • 利用8种口腔菌群和7种粪便菌群对人群是否感染COVID-19进行诊断,效果良好,不同区域的人群中诊断效能高达87.24%,并且成功将IgG阳性的疑似病人诊断为CP,诊断效能达92.11%;
  • 与CP相比,确诊并恢复的患者中有47个脂质分子减少,122个脂质分子富集。
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章台柳
郑州大学第一附属医院的任志刚和余祖江团队与浙江大学李兰娟院士的合作在Gut上发表文章,对COVID-19活跃性和恢复的患者的口腔、肠道微生物组和血清脂质组进行研究,发现诊断患者的口腔和肠道微生物群落的多样性减少,可使用口腔和肠道的标志性细菌作为非侵入式方式对病人进行诊断,效果良好。同时揭示出确诊患者和恢复患者的血清脂质组学变化,对微生物组与脂质组之间的相关性进行探索。
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Gut [IF:31.793]

Alterations in the human oral and gut microbiomes and lipidomics in COVID-19

COVID-19导致的人类口腔和肠道微生物组学及脂质组学改变

10.1136/gutjnl-2020-323826

2021-03-31, Article

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Objective: To characterise the oral microbiome, gut microbiome and serum lipid profiles in patients with active COVID-19 and recovered patients; evaluate the potential of the microbiome as a non-invasive biomarker for COVID-19; and explore correlations between the microbiome and lipid profile.
Design: We collected and sequenced 392 tongue-coating samples, 172 faecal samples and 155 serum samples from Central China and East China. We characterised microbiome and lipid molecules, constructed microbial classifiers in discovery cohort and verified their diagnostic potential in 74 confirmed patients (CPs) from East China and 37 suspected patients (SPs) with IgG positivity.
Results: Oral and faecal microbial diversity was significantly decreased in CPs versus healthy controls (HCs). Compared with HCs, butyric acid-producing bacteria were decreased and lipopolysaccharide-producing bacteria were increased in CPs in oral cavity. The classifiers based on 8 optimal oral microbial markers (7 faecal microbial markers) achieved good diagnostic efficiency in different cohorts. Importantly, diagnostic efficacy reached 87.24% in the cross-regional cohort. Moreover, the classifiers successfully diagnosed SPs with IgG antibody positivity as CPs, and diagnostic efficacy reached 92.11% (98.01% of faecal microbiome). Compared with CPs, 47 lipid molecules, including sphingomyelin (SM)(d40:4), SM(d38:5) and monoglyceride(33:5), were depleted, and 122 lipid molecules, including phosphatidylcholine(36:4p), phosphatidylethanolamine (PE)(16:0p/20:5) and diglyceride(20:1/18:2), were enriched in confirmed patients recovery.
Conclusion: This study is the first to characterise the oral microbiome in COVID-19, and oral microbiomes and lipid alterations in recovered patients, to explore their correlations and to report the successful establishment and validation of a diagnostic model for COVID-19.

First Authors:
Zhigang Ren,Haiyu Wang,Guangying Cui,Haifeng Lu

Correspondence Authors:
Zhigang Ren,Zujiang Yu,Lanjuan Li

All Authors:
Zhigang Ren,Haiyu Wang,Guangying Cui,Haifeng Lu,Ling Wang,Hong Luo,Xinhua Chen,Hongyan Ren,Ranran Sun,Wenli Liu,Xiaorui Liu,Chao Liu,Li Ang,Xuemei Wang,Benchen Rao,Chengyu Yuan,Hua Zhang,Jiarui Sun,Xiaolong Chen,Bingjie Li,Chuansong Hu,Zhongwen Wu,Zujiang Yu,Quancheng Kan,Lanjuan Li

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