仁济医院:两种硬化性胆管炎中,粪便菌群和代谢组的改变和差异
创作:mildbreeze 审核:mildbreeze 07月01日
  • 纳入34例IgG4-SC、37例PSC和64例健康对照,进行多组学整合分析;
  • 2种疾病都呈现菌群的α多样性降低和组成改变,伴随粪便代谢组的变化,PSC尤为明显;
  • 2种疾病也存在不同的菌群和代谢组特征,基于代谢物的模型在疾病状态预测方面优于基于菌群的模型;
  • IgG4-SC的疾病相关菌属和代谢物多与转氨酶相关,Blautia的减少和琥珀酸的升高可能参与肝脏炎症;
  • PSC的菌群/代谢物特征与胆汁淤积表型相关,例如真杆菌属和次级胆汁酸的共同减少。
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mildbreeze
IgG4相关硬化性胆管炎(IgG4-SC)和原发性硬化性胆管炎(PSC)有着相似的临床表现,但有着不同的治疗和预后。已有研究表明肠道菌群改变可能参与了PSC病理,但菌群在IgG4-SC中的作用尚缺乏研究。上海交通大学医学院附属仁济医院的Ruqi Tang、马雄、Min Lian与团队,近期在Gut发表研究,通过对IgG4-SC、PSC和健康人进行肠道微生物组、代谢组和人体表型进行整合多组学分析,揭示了两种疾病在宿主-微生物互作中的相似和不同之处,为深入探索发病机理提供了新线索。
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Gut [IF:23.059]

Altered faecal microbiome and metabolome in IgG4-related sclerosing cholangitis and primary sclerosing cholangitis

IgG4相关硬化性胆管炎和原发性硬化性胆管炎的粪便微生物组和代谢组改变

10.1136/gutjnl-2020-323565

05-25, Article

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OBJECTIVE: Multiple clinical similarities exist between IgG4-related sclerosing cholangitis (IgG4-SC) and primary sclerosing cholangitis (PSC), and while gut dysbiosis has been extensively studied in PSC, the role of the gut microbiota in IgG4-SC remains unknown. Herein, we aimed to evaluate alterations of the gut microbiome and metabolome in IgG4-SC and PSC.
DESIGN: We performed 16S rRNA gene amplicon sequencing of faecal samples from 135 subjects with IgG4-SC (n=34), PSC (n=37) and healthy controls (n=64). A subset of the samples (31 IgG4-SC, 37 PSC and 45 controls) also underwent untargeted metabolomic profiling.
RESULTS: Compared with controls, reduced alpha-diversity and shifted microbial community were observed in IgG4-SC and PSC. These changes were accompanied by differences in stool metabolomes. Importantly, despite some common variations in the microbiota composition and metabolic activity, integrative analyses identified distinct host-microbe associations in IgG4-SC and PSC. The disease-associated genera and metabolites tended to associate with the transaminases in IgG4-SC. Notable depletion of and elevated succinic acid may underlie hepatic inflammation in IgG4-SC. In comparison, potential links between the microbial or metabolic signatures and cholestatic parameters were detected in PSC. Particularly, concordant decrease of and microbiota-derived metabolites, including secondary bile acids, implicated novel host-microbial metabolic pathways involving cholestasis of PSC. Interestingly, the predictive models based on metabolites were more effective in discriminating disease status than those based on microbes.
CONCLUSIONS: Our data reveal that IgG4-SC and PSC possess divergent host-microbe interplays that may be involved in disease pathogenesis. These data emphasise the uniqueness of IgG4-SC.

First Authors:
Qiaoyan Liu,Bo Li,Yikang Li

Correspondence Authors:
Min Lian,Xiong Ma,Ruqi Tang

All Authors:
Qiaoyan Liu,Bo Li,Yikang Li,Yiran Wei,Bingyuan Huang,Jubo Liang,Zhengrui You,You Li,Qiwei Qian,Rui Wang,Jun Zhang,Ruiling Chen,Zhuwan Lyu,Yong Chen,Mingxia Shi,Xiao Xiao,Qixia Wang,Qi Miao,Jing-Yuan Fang,Merrill Eric Gershwin,Min Lian,Xiong Ma,Ruqi Tang

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