印遇龙院士、尹佳等:猪奶中的潜在益生菌
创作:mildbreeze 审核:mildbreeze 06月02日
  • 部分母乳微生物可以在母乳喂养期间从母体垂直传递给后代;
  • 用培养组学大规模分离和鉴定猪奶细菌,共筛选出1240株分离菌,可归为271个非冗余细菌类群;
  • 通过体外拮抗病原菌试验和果蝇百草枯模型抗氧化测试,筛选出一株戊糖片球菌SMM914;
  • 给哺乳仔猪灌服SMM914,于断奶7天后采样,发现SMM914使结肠中的有益菌乳杆菌属/科的相对丰度显著增加,并激活仔猪肝脏中Nrf2-Keap1抗氧化信号通路,以及改善血浆中的氨基酸代谢和脂质代谢通路。
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mildbreeze
母乳菌群可能是后代肠道菌群的一个重要来源。Microbiome最新发表了中科院亚热带生态所印遇龙院士和湖南师范大学尹佳与团队的研究,王乐莅博士为第一作者。该研究通过培养组学方法以及体外和果蝇模型筛选,从猪奶菌群中鉴定出一株有抗致病菌和抗氧化作用的潜在益生菌——戊糖片球菌SMM914,并在仔猪模型中对其有益功效进行了验证。该成果具有转化应用前景。
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Microbiome [IF:16.837]

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets

来自猪奶细菌的戊糖片球菌菌株在断奶仔猪中的抗氧化潜力

10.1186/s40168-022-01278-z

06-01, Article

Abstract & Authors:展开

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Background: In modern animal husbandry, breeders pay increasing attention to improving sow nutrition during pregnancy and lactation to favor the health of neonates. Sow milk is a main food source for piglets during their first three weeks of life, which is not only a rich repository of essential nutrients and a broad range of bioactive compounds, but also an indispensable source of commensal bacteria. Maternal milk microorganisms are important sources of commensal bacteria for the neonatal gut. Bacteria from maternal milk may confer a health benefit on the host.
Methods: Sow milk bacteria were isolated using culturomics followed by identification using 16S rRNA gene sequencing. To screen isolates for potential probiotic activity, the functional evaluation was conducted to assess their antagonistic activity against pathogens in vitro and evaluate their resistance against oxidative stress in damaged Drosophila induced by paraquat. In a piglet feeding trial, a total of 54 newborn suckling piglets were chosen from nine sows and randomly assigned to three treatments with different concentrations of a candidate strain. Multiple approaches were carried out to verify its antioxidant function including western blotting, enzyme activity analysis, metabolomics and 16S rRNA gene amplicon sequencing.
Results: The 1240 isolates were screened out from the sow milk microbiota and grouped into 271 bacterial taxa based on a nonredundant set of 16S rRNA gene sequencing. Among 80 Pediococcus isolates, a new Pediococcus pentosaceus strain (SMM914) showed the best performance in inhibition ability against swine pathogens and in a Drosophila model challenged by paraquat. Pretreatment of piglets with SMM914 induced the Nrf2-Keap1 antioxidant signaling pathway and greatly affected the pathways of amino acid metabolism and lipid metabolism in plasma. In the colon, the relative abundance of Lactobacillus was significantly increased in the high dose SMM914 group compared with the control group.
Conclusion: P. pentosaceus SMM914 is a promising probiotic conferring antioxidant capacity by activating the Nrf2-Keap1 antioxidant signaling pathway in piglets. Our study provided useful resources for better understanding the relationships between the maternal microbiota and offspring.

First Authors:
Leli Wang

Correspondence Authors:
Jia Yin,Yulong Yin

All Authors:
Leli Wang,Qihang Liu,Yuwei Chen,Xinlei Zheng,Chuni Wang,Yining Qi,Yachao Dong,Yue Xiao,Cang Chen,Taohong Chen,Qiuyun Huang,Zongzhao Zhai,Cimin Long,Huansheng Yang,Jianzhong Li,Lei Wang,Gaihua Zhang,Peng Liao,Yong-Xin Liu,Peng Huang,Jialu Huang,Qiye Wang,Huanhuan Chu,Jia Yin,Yulong Yin

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