印遇龙等:microRNA表达降低或会引起断奶仔猪腹泻
创作:九卿臣 审核:九卿臣 07月02日
  • 断奶应激会导致腹泻仔猪肠道形态受损、炎症反应增强、肠道菌群、代谢物和miRNAs组成显著改变;
  • ssc-miRNA-425-5p和ssc-miRNA-423-3p缺失导致普氏菌属丰度增加,富马酸还原酶表达升高,琥珀酸在肠道积累,导致腹泻;
  • 富集的琥珀酸会调节氯离子分泌导致肠上皮细胞液体分泌增多,也会激活MyD88依赖的TLR4信号通路增加炎症反应;
  • 机制上,miRNAs表达降低导致菌群产生的琥珀酸在肠道内富集,促进肠上皮细胞液体分泌和炎症反应,引起腹泻。
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九卿臣
腹泻是猪场常见的疾病,腹泻防控已经成为断奶仔猪饲养管理的关键。但仔猪腹泻的诱因复杂多样,相关机制仍需探究阐明。近日,中科院亚热带农业生态研究所的何流琴、印遇龙、李凤娜及团队在Gut Microbes发表最新研究,发现miRNAs表达减少会引起普氏菌属丰度增加,富马酸还原酶表达升高,琥珀酸在肠道积累,进而促进肠上皮细胞液体分泌和炎症反应,引起腹泻。总之,该研究为未来仔猪腹泻防控提供了新的理论依据。
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Gut Microbes [IF:9.434]

Intestinal accumulation of microbiota-produced succinate caused by loss of microRNAs leads to diarrhea in weanling piglets

microRNA缺失会引起菌群产生的琥珀酸在肠道内积聚导致断奶仔猪腹泻

10.1080/19490976.2022.2091369

06-26, Article

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Diarrheal disease is a common health problem with complex causality. Although diarrhea is accompanied by disturbances in microbial diversity, how gut microbes are involved in the occurrence of diarrhea remains largely unknown. Here, using a pig model of post-weaning stress-induced diarrhea, we aim to elucidate and enrich the mechanistic basis of diarrhea. We found significant alterations in fecal microbiome, their metabolites, and microRNAs levels in piglets with diarrhea. Specifically, loss of ssc-miRNA-425-5p and ssc-miRNA-423-3p, which inhibit the gene expression of fumarate reductase (frd) in Prevotella genus, caused succinate accumulation in piglets, which resulted in diarrhea. Single-cell RNA sequencing indicated impaired epithelial function and increased immune response in the colon of piglet with diarrhea. Notably, the accumulated succinate increased colonic fluid secretion by regulating transepithelial Cl-secretion in the epithelial cells. Meanwhile, succinate promoted colonic inflammatory responses by activating MyD88-dependent TLR4 signaling in the macrophages. Overall, our findings expand the mechanistic basis of diarrhea and suggest that colonic accumulation of microbiota-produced succinate caused by loss of miRNAs leads to diarrhea in weanling piglets.

First Authors:
Xihong Zhou

Correspondence Authors:
Liuqin He,Yulong Yin,Fengna Li

All Authors:
Xihong Zhou,Yonghui Liu,Xia Xiong,Jingqing Chen,Wenjie Tang,Liuqin He,Zhigang Zhang,Yulong Yin,Fengna Li

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