谢鹏+魏泓:菌群相关抑郁症中的肠-脑轴蛋白质组学分析
  • 给无菌小鼠移植来自重度抑郁症患者的肠道菌群,可使小鼠出现抑郁样表型;
  • 定量蛋白质组学分析表明,这些小鼠的前额叶皮层(PFC)、肝脏、盲肠和血清中的蛋白质水平发生改变,且PFC和肝脏的蛋白质表达变化模式与慢性应急模型的不同;
  • 分析菌群相关的肠-脑轴蛋白质模块表明,肠-脑轴的蛋白质变化涉及代谢过程和炎症反应等多种生物学功能,其中能量代谢是蛋白质网络的核心变化。
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mildbreeze
肠道菌群可能通过肠-脑轴影响抑郁症的发生发展。重庆医科大学谢鹏团队和第三军医大学魏泓团队合作,近期在Translational Psychiatry发表研究,在移植了抑郁症患者肠道菌群的小鼠模型中,揭示了肠道菌群失调相关的肠-脑轴蛋白质表达和功能的改变,为进一步理解菌群在抑郁症中的作用带来了新启示。
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Proteomics analysis of the gut-brain axis in a gut microbiota-dysbiosis model of depression

抑郁症肠道菌群失调模型中的肠-脑轴的蛋白质组学分析

10.1038/s41398-021-01689-w

2021-11-08, Article

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Major depressive disorder (MDD) is a serious mental illness. Increasing evidence from both animal and human studies suggested that the gut microbiota might be involved in the onset of depression via the gut-brain axis. However, the mechanism in depression remains unclear. To explore the protein changes of the gut-brain axis modulated by gut microbiota, germ-free mice were transplanted with gut microbiota from MDD patients to induce depression-like behaviors. Behavioral tests were performed following fecal microbiota transplantation. A quantitative proteomics approach was used to examine changes in protein expression in the prefrontal cortex (PFC), liver, cecum, and serum. Then differential protein analysis and weighted gene coexpression network analysis were used to identify microbiota-related protein modules. Our results suggested that gut microbiota induced the alteration of protein expression levels in multiple tissues of the gut-brain axis in mice with depression-like phenotype, and these changes of the PFC and liver were model specific compared to chronic stress models. Gene ontology enrichment analysis revealed that the protein changes of the gut-brain axis were involved in a variety of biological functions, including metabolic process and inflammatory response, in which energy metabolism is the core change of the protein network. Our data provide clues for future studies in the gut-brain axis on protein level and deepen the understanding of how gut microbiota cause depression-like behaviors.

First Authors:
Yiyun Liu,Haiyang Wang,Siwen Gui,Benhua Zeng,Juncai Pu

Correspondence Authors:
Hong Wei,Peng Xie

All Authors:
Yiyun Liu,Haiyang Wang,Siwen Gui,Benhua Zeng,Juncai Pu,Peng Zheng,Li Zeng,Yuanyuan Luo,You Wu,Chanjuan Zhou,Jinlin Song,Ping Ji,Hong Wei,Peng Xie

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