木枣:多糖具有中等抗氧化活性

本文采用乙醇/硫酸铵体系作为多相溶剂,研究超声波辅助双水相萃取对枣多糖的影响。
通过单因素实验和响应面法得到的优化条件为:29%乙醇和15%硫酸铵双水相体系,萃取时间38分钟,温度48°C、液料比1:30,微波功率70w,提取率为8.18%。
通过紫外、红外光谱和扫描电镜对其结构进行表征;化学分析表明,枣多糖由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖、半乳糖醛酸组成。
DPPH和ABT试验表明,枣多糖具有中等抗氧化活性。
延伸阅读

Extraction and physicochemical properties of polysaccharides from Ziziphus Jujuba cv. Muzao by ultrasound-assisted aqueous two-phase extraction

枣多糖的超声波辅助、双水相萃取提取及理化性质。

10.1016/j.ijbiomac.2017.12.042

2017-12-09, Article

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In this paper, ultrasound-assisted aqueous two-phase extraction (UAATPE) performed via a one-step procedure was applied to extract polysaccharides from Ziziphus Jujuba cv. Muzao. Using an ethanol/ammonium sulfate system as a multiphase solvent, effects of UAATPE on extraction of ZMP, such as solvent-to-material ratio, composition of aqueous two-phase system, and extraction time and temperature, were investigated using response surface methodology on basis of results of single-factor experiment. The final optimal conditions were ATPS composition: 29% ethanol and 15% (NH4)2SO4, extraction time: 38min, extraction temperature: 48°C, solvent-to-material ratio: 30, and microwave power: 70W. Under these conditions, the experimental extraction yield was 8.18%. Chemical analysis revealed that ZMP were composed of rhamnose, arabinose, xylose, mannose, glucose, galactose, and galacturonic acid, at a ratio of 1.46:2.47:2.27:1.12:1.00:1.57:5.40, respectively. The structures were also characterized with UV, FTIR, and SEM. The DPPH and ABTS radical scavenging activities showed ZMP had moderately antioxidant activity.

First Authors:
Xiaolong Ji

Correspondence Authors:
Min Wang

All Authors:
Xiaolong Ji,Qiang Peng,Yuepeng Yuan,Fang Liu,Min Wang