Antioxidant and hepatoprotective effects of A. cerana honey against acute alcohol-induced liver damage in mice
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A. cerana honey, gathered from Apis cerana Fabricius (A. cerana), has not been fully studied. Samples of honey originating from six geographical regions (mainly in the Qinling Mountains of China) were investigated to determine their antioxidant and hepatoprotective effects against acute alcohol-induced liver damage. The results showed that A. cerana honeys from the Qinling Mountains had high total phenolic contents (345.1-502.1mgGAkg(-1)), ascorbic acid contents (153.8-368.4mgkg(-1)), and strong antioxidant activities in DPPH radical scavenging activity assays (87.5-136.2IC50mgmL(-1)), ferric reducing antioxidant powers (191.8-317.4mgTroloxkg(-1)), and ferrous ion-chelating activities (27.5-35.5mgNa2EDTAkg(-1)). Pretreatment with A. cerana honey (Qinling Mountains) at 5, 10, or 20gkg(-1) twice daily for 12weeks significantly inhibited serum lipoprotein oxidation and increased serum radical absorbance capacity (ORAC) (P<0.05). Moreover, A. cerana honey inhibited acute alcohol-induced increases in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum (P<0.05), reduced the production of hepatic malondialdehyde (MDA) (P<0.05), and promoted superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities (P<0.05). More importantly, it also remarkably inhibited the level of TGF-β1 in the serum and liver (P<0.05). The results of this study indicate that administration of A. cerana honey prevents acute alcohol-induced liver damage likely because of its antioxidant properties and ability to prevent oxidative stress.
Liming Wu,Wei Cao
Haoan Zhao,Ni Cheng,Liangliang He,Guoxia Peng,Xiaofeng Xue,Liming Wu,Wei Cao