Science:使用纳米孔测序平台实现拉沙热病毒的移动检测

创作:女巫 审核:高春辉 01月11日
2018年尼日利亚拉沙热出现有史以来最大的病例激增,引发对可能出现了传播能力更强的新毒株的担忧;
在疫情爆发期间,使用纳米孔测序对患者样本进行宏基因组测序,快速响应了民众和WHO的关切,有效的应对了这场危机;
通过分析36个病毒基因组并在120个样本中确认,发现此次爆发是由流行性拉沙病毒的多样性推动的,而非出现了新的毒株;
病毒传播途径是人畜传播,而非人与人的传播,因此应对疫情的关键是加强啮齿动物控制。
延伸阅读
Science [IF:41.037]

Metagenomic sequencing at the epicenter of the Nigeria 2018 Lassa fever outbreak

在尼日利亚2018年拉沙热暴发中心进行宏基因组测序

01-04, Report, 10.1126/science.aau9343more

Abstract:
The 2018 Nigerian Lassa fever season saw the largest ever recorded upsurge of cases, raising concerns over the emergence of a strain with increased transmission rate. To understand the molecular epidemiology of this upsurge, we performed, for the first time at the epicenter of an unfolding outbreak, metagenomic nanopore sequencing directly from patient samples, an approach dictated by the highly variable genome of the target pathogen. Genomic data and phylogenetic reconstructions were communicated immediately to Nigerian authorities and the World Health Organization to inform the public health response. Real-time analysis of 36 genomes and subsequent confirmation using all 120 samples sequenced in the country of origin revealed extensive diversity and phylogenetic intermingling with strains from previous years, suggesting independent zoonotic transmission events and thus allaying concerns of an emergent strain or extensive human-to-human transmission.

First Authors:
E Ogbaini-Emovon,S Günther,S Duraffour

Correspondence Authors:
S Günther

All Authors:
L E Kafetzopoulou,S T Pullan,P Lemey,M A Suchard,D U Ehichioya,M Pahlmann,A Thielebein,J Hinzmann,L Oestereich,D M Wozniak,K Efthymiadis,D Schachten,F Koenig,J Matjeschk,S Lorenzen,S Lumley,Y Ighodalo,D I Adomeh,T Olokor,E Omomoh,R Omiunu,J Agbukor,B Ebo,J Aiyepada,P Ebhodaghe,B Osiemi,S Ehikhametalor,P Akhilomen,M Airende,R Esumeh,E Muoebonam,R Giwa,A Ekanem,G Igenegbale,G Odigie,G Okonofua,R Enigbe,J Oyakhilome,E O Yerumoh,I Odia,C Aire,M Okonofua,R Atafo,E Tobin,D Asogun,N Akpede,P O Okokhere,M O Rafiu,K O Iraoyah,C O Iruolagbe,P Akhideno,C Erameh,G Akpede,E Isibor,D Naidoo,R Hewson,J A Hiscox,R Vipond,M W Carroll,C Ihekweazu,P Formenty,S Okogbenin,E Ogbaini-Emovon,S Günther,S Duraffour