遗传背景可抵抗菌群改变

创作:MAY DAY 审核:mildbreeze 09月29日
易患1型糖尿病(T1D)的小鼠(NOD)与不易患T1D的小鼠进行同居实验,让肠道菌在小鼠间转移;
从出生开始同居的NOD小鼠,肠道菌群变化适中(该变化随同居终止而减少),断奶后同居则菌群几乎无变化,早期干预更容易引起菌群改变;
同居对NOD鼠的糖尿病发作、肠道炎症和调节性T细胞比例无影响,但胰岛素特异性CD8+ T细胞比例降低,表明菌群有出抗原表位特异性的调节作用;
易患T1D的遗传背景能够抵抗不易患T1D的小鼠肠道菌群的影响。
延伸阅读

Early-life exposure to gut microbiota from disease protected mice does not impact disease outcome in type 1 diabetes susceptible NOD mice

生命早期暴露于疾病保护性小鼠的肠道菌群对于易患1型糖尿病的NOD小鼠的疾病发展没有影响

09-07, Article, 10.1111/imcb.12201more

Abstract:
The microbial community making up the gut microbiota can profoundly influence intestinal homeostasis and immune system development, and is believed to influence the development of complex diseases including type 1 diabetes (T1D). T1D susceptible non-obese diabetic (NOD) mice have been shown to harbour a distinct microbiota to disease protected mice. We hypothesised that the T1D susceptible genetic background of NOD mice would be resistant to the introduction of a C57BL/6 derived microbiota. NOD and C57BL/6 mice were cohoused either continually from birth, from birth until weaning or from weaning onwards, allowing transfer of microbiota between the mice. Cohousing NOD with C57BL/6 mice from before birth, resulted in moderate changes to the gut microbiota, whereas initiating co-housing at weaning only led to minimal changes. Terminating cohousing at weaning reduced the changes in the microbiota composition. However, diabetes onset was not significantly delayed and there was no reduction in intestinal inflammation or the proportion of regulatory T cells in the co-housed NOD mice. However, insulin but not IGRP-specific CD8+ T cells were reduced by co-housing suggesting an epitope-specific modulation of the autoreactive response by the gut microbiota. These results suggest that the T1D susceptible genetic background of the NOD mouse was resistant to the introduction of a C57BL/6 derived microbiota. This article is protected by copyright. All rights reserved.

First Authors:
Jane A Mullaney

Correspondence Authors:
Emma E Hamilton-Williams

All Authors:
Jane A Mullaney,Juliette E Stephens,Brooke E Geeling,Emma E Hamilton-Williams