肠道菌群对早期发育和孕晚期的代谢适应

创作:女巫 审核:mildbreeze 12月06日
通过宏转录组测序分析婴儿出生后第一年、其母亲分娩前几天和分娩后第一年的肠道菌群中的基因表达;
随着婴儿发育,菌群宏转录组中需氧代谢的标志消失,而与碳水化合物转运和代谢相关的功能表达增加,并变得多样化,与非孕妇的情况接近;
丁酸合成酶在3月龄时过表达,尽管此时大多数产丁酸微生物在肠道中丰度不高;
孕晚期肠道菌群的碳水化合物相关功能基因表达改变,可能体现了对该时期常见的血糖升高的适应性。
延伸阅读
EBioMedicine [IF:6.183]

Metabolic adaptation in the human gut microbiota during pregnancy and the first year of life

妊娠期和出生后第一年人体肠道菌群的代谢适应

11-08, Article, 10.1016/j.ebiom.2018.10.071more

Abstract:
BACKGROUND: The relationship between the gut microbiome and the human host is dynamic and we may expect adjustments in microbiome function if host physiology changes. Metatranscriptomic approaches should be key in unraveling how such adjustments occur.
METHODS: We employ metatranscriptomic sequencing analyses to study gene expression in the gut microbiota of infants through their first year of life, and of their mothers days before delivery and one year afterwards.
FINDINGS: In infants, hallmarks of aerobic metabolism disappear from the microbial metatranscriptome as development proceeds, while the expression of functions related to carbohydrate transport and metabolism increases and diversifies, approaching that observed in non-pregnant women. Butyrate synthesis enzymes are overexpressed at three months of age, even though most butyrate-producing organisms are still rare. In late pregnancy, the microbiota readjusts the expression of carbohydrate-related functions in a manner consistent with a high availability of glucose.
INTERPRETATION: Our findings suggest that butyrate production may be ensured in the gut of young infants before the typical butyrate synthesizers of the adult gut become abundant. The late pregnancy gut microbiota may be able to access the high levels of blood glucose characteristic of this period. Moreover, late pregnancy gut bacteria may reach stationary phase, which may affect their likelihood of translocating across the intestinal epithelium.
FUNDS: This work was supported by grants CSD2009-00006 (CONSOLIDER Program) and SAF2009-13032-C02-02 from MICINN (Ministry of Science and Innovation, Spain), and by grant SAF2012-31187 from MINECO (Ministry of Economics and Competitiveness, Spain).

First Authors:
María José Gosalbes

Correspondence Authors:
M Pilar Francino

All Authors:
María José Gosalbes,Joan Compte,Silvia Moriano-Gutierrez,Yvonne Vallès,Nuria Jiménez-Hernández,Xavier Pons,Alejandro Artacho,M Pilar Francino