【食品营养】肠道菌群对生物碱做了啥?
  • 本文采用大鼠肠道菌群与8种天然植物来源的异喹啉生物碱共培养的方法,并应用LC/MS(n)-IT-TOF技术鉴定代谢产物。
  • 黄藤素,延胡索乙素、蝙蝠葛碱和含有硝基六元异喹啉环的粉防己碱易被肠道菌群改变,并检测到9种脱甲基代谢物。
  • 但是,含含苯并氮杂的青藤碱、高三尖杉酯碱、 三尖杉酯碱和雪花莲胺碱,不能去甲基化。
  • 计算机辅助对接试验表明:生物碱与甾醇14α-去甲基酶的结合主要靠疏水作用,苯并氮杂结构产生的位阻减弱了结合作用。
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Molecules [IF:3.06]

In Vitro Study of the Metabolic Characteristics of Eight Isoquinoline Alkaloids from Natural Plants in Rat Gut Microbiota

8种天然植物来源的异喹啉生物碱在体外大鼠肠道菌群中的代谢特征研究

10.3390/molecules22060932

2017-06-04, Article

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Gut microbiota is populated with an immense number of microorganisms, which can be regulated by dietary components and drugs to markedly affect the nutritional and health status of the host. Eight medicinal isoquinoline alkaloids from natural plants were cultured anaerobically with rat gut microbiota and an LC/MS(n)-IT-TOF technique was used to identify the resulting metabolites. Palmatine, tetrahydropalmatine, dauricine, and tetrandrine containing nitro-hexatomic isoquinoline rings could be easily transformed by the intestinal flora in vitro and a total of nine demethylated metabolites were detected. However, sinomenine, homoharringtonine, harringtonine, and galanthamine, which all contained benzazepine, could not undergo demethylation. Computer-assisted docking was used to analyze the binding between these compounds and sterol 14α-demethylase. The computational results demonstrated that hydrophobic interactions were the main driving force for binding, but the steric hindrance produced by the benzazepine structure resulted in a weak interaction between the hit compounds and the enzyme. This work illustrated that gut microbiota were important in the metabolism of isoquinoline alkaloids.

First Authors:
Chi-Yu He,Jie Fu

Correspondence Authors:
Yan Wang,Jian-Dong Jiang

All Authors:
Chi-Yu He,Jie Fu,Jia-Wen Shou,Zhen-Xiong Zhao,Long Ren,Yan Wang,Jian-Dong Jiang

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