肠型影响免疫细胞亚群及IgA反应性
创作:沈志勋 审核:沈志勋 03月12日
  • 选取35名健康人,分析肠道菌群组成、血液中的B细胞及T细胞亚群、粪便及血浆IgA与不同肠道细菌的结合;
  • 鉴定出3种不同类型肠型,1型与2型分别以拟杆菌属及普氏菌属为主,3型的细菌多样性及丰度显著高于1/2型,并以Ruminococacceae为主;
  • 3型的Th17及Th22细胞显著减少,1型的Th17.1细胞显著增加;
  • 不同肠型的血浆及粪便IgA与不同肠道细菌的结合模式相似,血浆IgA的结合水平更高,血浆IgA结合长双歧杆菌的水平受到肠型影响。
延伸阅读

Differences in systemic IgA reactivity and circulating Th subsets in healthy volunteers with specific microbiota enterotypes

不同类型肠型的健康志愿者中系统性IgA反应性及循环Th细胞亚群的差异

10.3389/fimmu.2019.00341

03-07, Article

Abstract & Authors:展开

Abstract:收起
Changes in the intestinal microbiota have been associated with the development of immune-mediated diseases in humans. Additionally, the introduction of defined bacterial species into the mouse intestinal microbiota has been shown to impact on the adaptive immune response. However, how much impact the intestinal microbiota composition actually has on regulating adaptive immunity remains poorly understood. Therefore, we studied aspects of the adaptive immunity in healthy adults possessing distinct intestinal microbiota profiles. The intestinal microbiota composition was determined via Illumina sequencing of bacterial 16S rRNA genes extracted from the feces of 35 individuals. Blood B-cell and T-cell subsets from the same individuals were studied using flow cytometry. Finally, the binding of fecal and plasma Immunoglobulin A (IgA) to intestinal bacteria (associated with health and disease) Bacteroides fragilis, Prevotella copri, Bifidobacterium longum, Clostridium difficile, and Escherichia coli was analyzed using ELISA. Unsupervised clustering of microbiota composition revealed the presence of three clusters within the cohort. Cluster 1 and 2 were similar to previously-described enterotypes with a predominance of Bacteroides in Cluster 1 and Prevotella in Cluster 2. The bacterial diversity (Shannon index) and bacterial richness of Cluster 3 was significantly higher than observed in Clusters 1 and 2, with the Ruminococacceae tending to predominate. Within circulating B- and T-cell subsets, only Th subsets were significantly different between groups of distinct intestinal microbiota. Individuals of Cluster 3 have significantly fewer Th17 and Th22 circulating cells, while Th17.1 cell numbers were increased in individuals of Cluster 1. IgA reactivity to intestinal bacteria was higher in plasma than feces, and individuals of Cluster 1 had significant higher plasma IgA reactivity against B. longum than individuals of Cluster 2. In conclusion, we identified three distinct fecal microbiota clusters, of which two clusters resembled previously-described “enterotypes”. Global T-cell and B-cell immunity seemed unaffected, however, circulating Th subsets and plasma IgA reactivity were significantly different between Clusters. Hence, the impact of intestinal bacteria composition on human B cells, T cells and IgA reactivity appears limited in genetically-diverse and environmentally-exposed humans, but can skew antibody reactivity and Th cell subsets.

First Authors:
Christina Grosserichter-Wagener

Correspondence Authors:
Menno C van Zelm

All Authors:
Christina Grosserichter-Wagener,Djawad Radjabzadeh,Hessel van der Weide,Kyra N Smit,Robert Kraaij,John P Hays,Menno C van Zelm

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