胰腺外分泌功能与肠道菌群的组成及多样性相关

创作:沈志勋 审核:沈志勋 01月11日
对1795名健康志愿者的粪便样本进行测序分析,并检测粪便中的胰弹性蛋白酶水平;
胰弹性蛋白酶水平与肠道菌群的多样性显著相关;
对比胰腺外分泌功能减退(RPF)及胰腺外分泌功能正常(对照)的受试者,发现22个分类群的丰度存在显著差异,例如普氏菌属的增加及拟杆菌属的减少;
相比于对照受试者,RPF受试者的肠型从1型转向2型;
对分泌素刺激下的胰液分泌进行定量成像分析,发现胰液分泌的变化也与菌群多样性显著相关。
延伸阅读
Gastroenterology [IF:20.773]

Impaired Exocrine Pancreatic Function Associates With Changes in Intestinal Microbiota Composition and Diversity

胰腺外分泌功能受损与肠道菌群组成及多样性变化相关

2018-11-01, Article, 10.1053/j.gastro.2018.10.047more

Abstract:
BACKGROUND & AIMS: Changes in intestinal microbiome composition are associated with inflammatory, metabolic, and malignant disorders. We studied how exocrine pancreatic function affects intestinal microbiota.
METHODS: We performed 16S rRNA gene sequencing analysis of stool samples from 1795 volunteers from the population-based study of health in Pomerania who had no history of pancreatic disease. We also measured fecal pancreatic elastase by ELISA and performed quantitative imaging of secretin-stimulated pancreatic fluid secretion. Associations of exocrine pancreatic function with microbial diversity or individual genera were calculated by permutational analysis of variance or linear regression, respectively.
RESULTS: Differences in pancreatic elastase levels associated with significantly (P<.0001) greater changes in microbiota diversity than with participant age, body mass index, sex, smoking, alcohol consumption, or dietary factors. Significant changes in the abundance of thirty taxa, such as an increase in Prevotella (q<.0001) and a decrease of Bacteroides (q<.0001), indicated a shift from a type-1 to a type-2 enterotype. Changes in pancreatic fluid secretion alone were also associated with changes in microbial diversity (P=.0002), although to a lesser degree.
CONCLUSIONS: In an analysis of fecal samples from 1795 volunteers, pancreatic acinar cell, rather than duct cell, function is presently the single most significant host factor to be associated with changes in intestinal microbiota composition.

First Authors:
Fabian Frost

Correspondence Authors:
Markus M Lerch

All Authors:
Fabian Frost,Tim Kacprowski,Malte Rühlemann,Robin Bülow,Jens-Peter Kühn,Andre Franke,Femke-Anouska Heinsen,Maik Pietzner,Matthias Nauck,Uwe Völker,Henry Völzke,Ali A Aghdassi,Matthias Sendler,Julia Mayerle,Frank U Weiss,Georg Homuth,Markus M Lerch