Nature:芽孢杆菌益生菌清除致病菌的机制

创作:mildbreeze 审核:mildbreeze 11月01日
分析泰国农村的200个健康个体,发现肠内存在芽孢杆菌(主要是枯草芽孢杆菌)的人,肠道和鼻腔中没有金黄色葡萄球菌定殖;
小鼠实验表明,金黄色葡萄球菌需要Agr群体感应系统来实现肠道定殖,芽孢杆菌产生的脂肽芬荠素,与Agr群体感应中的关键因子AIP有相似结构,可干扰Agr信号转导,从而抑制Agr群体感应;
给小鼠灌胃枯草芽孢杆菌孢子,可完全消除金黄色葡萄球菌的肠道定殖,而不产生芬荠素的枯草芽孢杆菌无此作用。
延伸阅读
Nature [IF:41.577]

Pathogen elimination by probiotic Bacillus via signalling interference

芽孢杆菌益生菌通过信号干扰清除致病菌

10-10, Article, 10.1038/s41586-018-0616-ymore

Abstract:
Probiotic nutrition is frequently claimed to improve human health. In particular, live probiotic bacteria obtained with food are thought to reduce intestinal colonization by pathogens, and thus to reduce susceptibility to infection. However, the mechanisms that underlie these effects remain poorly understood. Here we report that the consumption of probiotic Bacillus bacteria comprehensively abolished colonization by the dangerous pathogen Staphylococcus aureus in a rural Thai population. We show that a widespread class of Bacillus lipopeptides, the fengycins, eliminates S. aureus by inhibiting S. aureus quorum sensing—a process through which bacteria respond to their population density by altering gene regulation. Our study presents a detailed molecular mechanism that underlines the importance of probiotic nutrition in reducing infectious disease. We also provide evidence that supports the biological significance of probiotic bacterial interference in humans, and show that such interference can be achieved by blocking a pathogen’s signalling system. Furthermore, our findings suggest a probiotic-based method for S. aureus decolonization and new ways to fight S. aureus infections.

First Authors:
Pipat Piewngam

Correspondence Authors:
Michael Otto

All Authors:
Pipat Piewngam,Yue Zheng,Thuan H Nguyen,Seth W Dickey,Hwang-Soo Joo,Amer E Villaruz,Kyle A Glose,Emilie L Fisher,Rachelle L Hunt,Barry Li,Janice Chiou,Sujiraphong Pharkjaksu,Sunisa Khongthong,Gordon Y C Cheung,Pattarachai Kiratisin,Michael Otto