食用北极浆果或改善肥胖小鼠代谢健康
创作:兵兵 审核:JIAN 剑 2018年06月22日
  • 高糖高脂饮食的小鼠分别口服沼泽蓝莓(BBE)/野生黄莓(CLE)/岩高兰(CRE)/越橘(LGE)提取物以及赋形对照(HFHS),共8周。
  • CLE, ABE 以及 LGE饲喂的小鼠,空腹和餐后高胰岛素血症得以改善; 与对照组相比,肝脏AKt磷酸化水平增加;与较低的肝脂肪、循环类毒素、肝脏/肠道炎症有关,也与主要肠道菌群变化有关。
  • 所有的浆果提取物都不影响C-反应蛋白表达,也不影响小鼠体重。
  • 结论:浆果有助于改善肥胖相关的慢性炎症反应及代谢异常。
关键字
延伸阅读本研究的原文信息和链接出处,以及相关解读和评论文章。欢迎读者朋友们推荐!
图片
Diabetologia [IF:7.113]

Arctic berry extracts target the gut-liver axis to alleviate metabolic endotoxaemia, insulin resistance and hepatic steatosis in diet-induced obese mice

北极浆果提取物以肠-肝轴为作用靶点来减轻食物诱导的肥胖小鼠的代谢内毒素血症,胰岛素抗性以及脂肪肝

10.1007/s00125-017-4520-z

2018-04-01, Article

Abstract & Authors:展开

Abstract:收起
AIMS/HYPOTHESIS: There is growing evidence that fruit polyphenols exert beneficial effects on the metabolic syndrome, but the underlying mechanisms remain poorly understood. In the present study, we aimed to analyse the effects of polyphenolic extracts from five types of Arctic berries in a model of diet-induced obesity.
METHODS: Male C57BL/6 J mice were fed a high-fat/high-sucrose (HFHS) diet and orally treated with extracts of bog blueberry (BBE), cloudberry (CLE), crowberry (CRE), alpine bearberry (ABE), lingonberry (LGE) or vehicle (HFHS) for 8 weeks. An additional group of standard-chow-fed, vehicle-treated mice was included as a reference control for diet-induced obesity. OGTTs and insulin tolerance tests were conducted, and both plasma insulin and C-peptide were assessed throughout the OGTT. Quantitative PCR, western blot analysis and ELISAs were used to assess enterohepatic immunometabolic features. Faecal DNA was extracted and 16S rRNA gene-based analysis was used to profile the gut microbiota.
RESULTS: Treatment with CLE, ABE and LGE, but not with BBE or CRE, prevented both fasting hyperinsulinaemia (mean ± SEM [pmol/l]: chow 67.2 ± 12.3, HFHS 153.9 ± 19.3, BBE 114.4 ± 14.3, CLE 82.5 ± 13.0, CRE 152.3 ± 24.4, ABE 90.6 ± 18.0, LGE 95.4 ± 10.5) and postprandial hyperinsulinaemia (mean ± SEM AUC [pmol/l × min]: chow 14.3 ± 1.4, HFHS 31.4 ± 3.1, BBE 27.2 ± 4.0, CLE 17.7 ± 2.2, CRE 32.6 ± 6.3, ABE 22.7 ± 18.0, LGE 23.9 ± 2.5). None of the berry extracts affected C-peptide levels or body weight gain. Levels of hepatic serine phosphorylated Akt were 1.6-, 1.5- and 1.2-fold higher with CLE, ABE and LGE treatment, respectively, and hepatic carcinoembryonic antigen-related cell adhesion molecule (CEACAM)-1 tyrosine phosphorylation was 0.6-, 0.7- and 0.9-fold increased in these mice vs vehicle-treated, HFHS-fed mice. These changes were associated with reduced liver triacylglycerol deposition, lower circulating endotoxins, alleviated hepatic and intestinal inflammation, and major gut microbial alterations (e.g. bloom of Akkermansia muciniphila, Turicibacter and Oscillibacter) in CLE-, ABE- and LGE-treated mice.
CONCLUSIONS/INTERPRETATION: Our findings reveal novel mechanisms by which polyphenolic extracts from ABE, LGE and especially CLE target the gut-liver axis to protect diet-induced obese mice against metabolic endotoxaemia, insulin resistance and hepatic steatosis, which importantly improves hepatic insulin clearance. These results support the potential benefits of these Arctic berries and their integration into health programmes to help attenuate obesity-related chronic inflammation and metabolic disorders.
DATA AVAILABILITY: All raw sequences have been deposited in the public European Nucleotide Archive server under accession number PRJEB19783 ( https://www.ebi.ac.uk/ena/data/view/PRJEB19783 ).

First Authors:
Fernando F Anhê

Correspondence Authors:
André Marette

All Authors:
Fernando F Anhê,Thibault V Varin,Mélanie Le Barz,Geneviève Pilon,Stéphanie Dudonné,Jocelyn Trottier,Philippe St-Pierre,Cory S Harris,Michel Lucas,Mélanie Lemire,Éric Dewailly,Olivier Barbier,Yves Desjardins,Denis Roy,André Marette

评论