青砖茶中的抗氧化活性多糖
  • 对青砖茶中分离的粗茶多糖(CTPS)和纯化分离出的两种多糖(TPS-1、TPS-2),分析结构和抗氧化活性。
  • 成分分析显示,粗茶多糖中的中性糖、糖醛酸、蛋白质和多酚含量差异显著; 红外光谱发现多糖具有相似的特征吸收峰。
  • 紫外-光谱、圆二色谱、扫描电子显微镜和热重分析表明,其蛋白质含量、表面特征、构象特征和热力学行为存在较大差异。
  • TPS-1、TPS-2体外抗氧化活性具有浓度依赖性,TPS-2比CTP和TPS-1显示出更好的抗氧化活性。
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Structural characterization and evaluation of the antioxidant activities of polysaccharides extracted from Qingzhuan brick tea

青砖茶中的活性多糖

10.1016/j.ijbiomac.2017.03.189

2017-08-01, Article

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The crude tea polysaccharides (CTPS) from Qingzhuan brick tea(QZBT) were extracted and fractionated to afford two fractions, namely TPS-1 and TPS-2. Analyses were conducted concerning the structural characterization and antioxidant activities of these samples. Component analysis revealed that the carbohydrate, uronic acid, protein and polyphenol contents of these samples differed significantly. Fourier transform infrared analysis showed that these samples showed similar characteristic absorption peaks for polysaccharides. Ultraviolet-visible spectroscopy, circular dichroism, scanning electron microscopy and thermogravimetric analyses indicated that there were considerable differences in the presence of protein, surface features, conformational characteristics and thermodynamic behaviors. For antioxidant activities in vitro, CTPS, TPS-1 and TPS-2 exhibited concentration-dependent antioxidant activities, with TPS-2 showing significantly higher antioxidant activity than CTPS and TPS-1. These results provide a scientific and strong foundation for the use of tea polysaccharides(TPS) from QZBT and further research towards the relationships between the characteristics and antioxidant activities of TPS.

First Authors:
Xinhe Yang

Correspondence Authors:
Xinhe Yang,Zhonghua Liu

All Authors:
Xinhe Yang,Mingjun Huang,Caiqin Qin,Bangyu Lv,Qingli Mao,Zhonghua Liu

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