侵袭性牙周炎患者及其亲属口腔菌群结构的探究

创作:陈峰 审核:蓝灿辉 2016年05月27日
侵袭性牙周炎患者龈下菌斑中相对丰度较高的P.gingivalis是最主要的牙周致病菌
AgP患者龈下菌群复杂程度弱于患重度慢性牙周炎的亲属
AgP患者龈下菌斑与唾液中牙周红色复合体的相对丰度显著相关
相对陌生牙周炎患者,AgP患者与亲属口腔菌群结构更相似。
延伸阅读

Oral microbiome in chinese patients with aggressive periodontitis and their family members

2015-11-14, Article, 10.1111/jcpe.12463more

Abstract:
AIM: To investigate the microbiome composition in Chinese patients with aggressive periodontitis (AgP), and to compare the similarity of bacterial profiles between AgP patients and their family members.
MATERIAL AND METHODS: Pooled subgingival plaque and saliva samples were collected from 10 AgP patients and 10 of their first-degree blood relatives with chronic periodontitis. DNA amplicons of the V1-V3 hypervariable region of the bacterial 16S rRNA gene were generated, and were subjected to 454-pyrosequencing.
RESULTS: In subgingival plaque, the unweighted UniFrac distances between family members were significantly lower than those in unrelated participants (p = 0.039). Compared with the relatives, the microbiota of subgingival plaque and saliva from AgP patients revealed significantly lower taxonomic diversity. High relative abundance of Porphyromonas gingivalis (about 35.88%) was detected in subgingival plaque from AgP patients. The relative abundance of P. gingivalis and Red complex pathogens (P. gingivalis, Treponema denticola and Tannerella forsythia) in the subgingival plaque and saliva samples from the same individual were significantly correlated in AgP patients (ρ= 0.687 and 0.678, respectively).
CONCLUSIONS: There is a kinship in the phylogenetic architecture of microbiota among Chinese AgP patients and their family members. P. gingivalis might be a predominant pathogen in these Chinese AgP patients.

All Authors:
Yi Li,Xianghui Feng,Li Xu,Li Zhang,Ruifang Lu,Dong Shi,Xiane Wang,Feng Chen,Jie Li,Huanxin Meng