1型糖尿病和MODY2糖尿病患儿的肠道菌群特征

创作:fang fang 审核:mildbreeze 10月08日
纳入15例1型糖尿病(T1D)、15例由单基因突变导致的MODY2型糖尿病和13例健康儿童,分析肠道菌群组成和功能;
MODY2和T1D患儿的肠道菌群多样性明显下降,菌群组成改变,T1D组中拟杆菌等5个菌属增多、双歧杆菌等4个菌属减少,MODY2组普氏菌属增多、瘤胃球菌和拟杆菌属减少;
MODY2和T1D患儿的肠道通透性增加,T1D组血清促炎细胞因子和脂多糖增多;
T1D组肠道菌群与脂质、氨基酸代谢等相关的基因比例明显增加,菌群功能差异明显。
延伸阅读
Diabetes Care [IF:13.397]

Gut Microbiota Differs in Composition and Functionality Between Children With Type 1 Diabetes, MODY2, and Healthy Control Subjects: A Case-Control Study

在1型糖尿病、青少年发病的成年型糖尿病和健康对照受试者中肠道菌群的组成和功能存在差异:一项病例对照研究

09-27, Article, 10.2337/dc18-0253more

Abstract:
OBJECTIVE: Type 1 diabetes is associated with compositional differences in gut microbiota. To date, no microbiome studies have been performed in maturity-onset diabetes of the young 2 (MODY2), a monogenic cause of diabetes. Gut microbiota of type 1 diabetes, MODY2, and healthy control subjects was compared.
RESEARCH DESIGN AND METHODS: This was a case-control study in 15 children with type 1 diabetes, 15 children with MODY2, and 13 healthy children. Metabolic control and potential factors modifying gut microbiota were controlled. Microbiome composition was determined by 16S rRNA pyrosequencing.
RESULTS: Compared with healthy control subjects, type 1 diabetes was associated with a significantly lower microbiota diversity, a significantly higher relative abundance of Bacteroides, Ruminococcus, Veillonella, Blautia, and Streptococcus genera, and a lower relative abundance of Bifidobacterium, Roseburia, Faecalibacterium, and Lachnospira. MODY2 showed a significantly higher Prevotella abundance and a lower Ruminococcus and Bacteroides abundance. Proinflammatory cytokines and lipopolisaccharides were increased in type 1 diabetes, and gut permeability (determined by zonulin levels) was significantly increased in type 1 diabetes and MODY2. The PICRUSt analysis found an increment of genes related to lipid and amino acid metabolism, ABC transport, lipopolysaccharide biosynthesis, arachidonic acid metabolism, antigen processing and presentation, and chemokine signaling pathways in type 1 diabetes.
CONCLUSIONS: Gut microbiota in type 1 diabetes differs at taxonomic and functional levels not only in comparison with healthy subjects but fundamentally with regard to a model of nonautoimmune diabetes. Future longitudinal studies should be aimed at evaluating if the modulation of gut microbiota in patients with a high risk of type 1 diabetes could modify the natural history of this autoimmune disease.

First Authors:
Isabel Leiva-Gea,Lidia Sánchez-Alcoholado

Correspondence Authors:
José Carlos Fernández-García

All Authors:
Isabel Leiva-Gea,Lidia Sánchez-Alcoholado,Beatriz Martín-Tejedor,Daniel Castellano-Castillo,Isabel Moreno-Indias,Antonio Urda Cardona,Francisco J Tinahones,José Carlos Fernández-García,María Isabel Queipo-Ortuño