噬菌体可有效防治霍乱

创作:可乐 审核:可乐 11月09日
采用单个噬菌体用于在幼兔模型中预防性和治疗性地控制霍乱;
在防治两端,噬菌体处理的动物均没有显示出霍乱临床症状;
噬菌体处理的动物肠道中的致病菌计数减少了4个数量级;
只有在接受霍乱弧菌感染的动物中才检测到噬菌体增殖;
没有从动物中分离出抗噬菌体的细菌突变体;
结论:首次证明单个噬菌体可以有效地治疗霍乱,而没有可检测到的耐药菌株出现。
延伸阅读

Reviving Phage Therapy for the Treatment of Cholera

噬菌体疗法治疗霍乱

11-03, Report, 10.1093/infdis/jiy563more

Abstract:
Cholera remains a major risk in developing countries, particularly after natural or man-made disasters. Vibrio cholerae El Tor is the most important cause of these outbreaks, and is becoming increasingly resistant to antibiotics, so alternative therapies are urgently needed. In this study, a single bacteriophage, Phi_1, was used to control cholera prophylactically and therapeutically in an infant rabbit model. In both cases, phage-treated animals showed no clinical signs of disease, compared with 69% of untreated control animals. Bacterial counts in the intestines of phage-treated animals were reduced by up to 4 log10 colony-forming units/g. There was evidence of phage multiplication only in animals that received a V. cholerae challenge. No phage-resistant bacterial mutants were isolated from the animals, despite extensive searching. This is the first evidence that a single phage could be effective in the treatment of cholera, without detectable levels of resistance. Clinical trials in human patients should be considered.

First Authors:
Sudhakar Bhandare

Correspondence Authors:
Robert J Atterbury

All Authors:
Sudhakar Bhandare,Joan Colom,Abiyad Baig,Jenny M Ritchie,Habib Bukhari,Muhammad A Shah,Banwarilal L Sarkar,Jingliang Su,Brendan Wren,Paul Barrow,Robert J Atterbury