• 利用质谱流式细胞技术对人体粘膜免疫系统进行高维分析;
  • 通过数据分析发现了一些未知的免疫细胞异质性;
  • 鉴定出了一些粘膜淋巴恶性肿瘤及其肿瘤细胞前体;
  • 通过数据可视化鉴定出了许多组织及疾病相关性的免疫亚群。
蓝灿辉 | 热心肠先生
Immunity [IF:21.522]

Mass Cytometry of the Human Mucosal Immune System Identifies Tissue- and Disease-Associated Immune Subsets



2016-05-17, Article

Abstract & Authors:展开

Inflammatory intestinal diseases are characterized by abnormal immune responses and affect distinct locations of the gastrointestinal tract. Although the role of several immune subsets in driving intestinal pathology has been studied, a system-wide approach that simultaneously interrogates all major lineages on a single-cell basis is lacking. We used high-dimensional mass cytometry to generate a system-wide view of the human mucosal immune system in health and disease. We distinguished 142 immune subsets and through computational applications found distinct immune subsets in peripheral blood mononuclear cells and intestinal biopsies that distinguished patients from controls. In addition, mucosal lymphoid malignancies were readily detected as well as precursors from which these likely derived. These findings indicate that an integrated high-dimensional analysis of the entire immune system can identify immune subsets associated with the pathogenesis of complex intestinal disorders. This might have implications for diagnostic procedures, immune-monitoring, and treatment of intestinal diseases and mucosal malignancies.

First Authors:
Vincent van Unen

Correspondence Authors:
Frits Koning

All Authors:
Vincent van Unen,Na Li,Ilse Molendijk,Mine Temurhan,Thomas Höllt,Andrea E van der Meulen-de Jong,Hein W Verspaget,M Luisa Mearin,Chris J Mulder,Jeroen van Bergen,Boudewijn P F Lelieveldt,Frits Koning