从乳制品及人粪便中分离出的2株潜在益生菌

创作:李珊珊 审核:沈志勋 01月11日
从乳制品和人粪便中分离出5种具有体外免疫调节活性的菌株,分别为植物乳杆菌Lb38、干酪乳杆菌L79、鼠李糖乳杆菌Lb102、动物双歧杆菌乳亚种Bf26和Bf141;
分别给予饮食诱导的肥胖小鼠上述菌株,干预8周;
Lb102和Bf141可降低饮食诱导的肥胖、内脏脂肪堆积和炎症,改善葡萄糖耐受和胰岛素敏感性,增强与肠道菌群组成选择性变化相关的肠道完整性标志物水平;
其它3种菌作用不明显;
Lb102和Bf141为具有改善肥胖和代谢紊乱功能的新益生菌。
延伸阅读
FASEB Journal [IF:5.391]

In vivo screening of multiple bacterial strains identifies Lactobacillus rhamnosus Lb102 and Bifidobacterium animalis ssp. lactis Bf141 as probiotics that improve metabolic disorders in a mouse model of obesity

对多菌株的体内筛选鉴定出可改善肥胖小鼠代谢紊乱的益生菌——鼠李糖乳杆菌Lb102和动物双歧杆菌乳亚种Bf141

2018-12-31, Article, 10.1096/fj.201801672Rmore

Abstract:
Given the growing evidence that gut dysfunction, including changes in gut microbiota composition, plays a critical role in the development of inflammation and metabolic diseases, the identification of novel probiotic bacteria with immunometabolic properties has recently attracted more attention. Herein, bacterial strains were first isolated from dairy products and human feces and then screened in vitro for their immunomodulatory activity. Five selected strains were further analyzed in vivo, using a mouse model of diet-induced obesity. C57BL/6 mice were fed a high-fat high-sucrose diet, in combination with 1 of 3 Lactobacillus strains (Lb38, L. plantarum; L79, L. paracasei/casei; Lb102, L. rhamnosus) or Bifidobacterium strains (Bf26, Bf141, 2 different strains of B. animalis ssp. lactis species) administered for 8 wk at 10 colony-forming units/d. Whereas 3 strains showed only modest (Lb38, Bf26) or no (L79) effects, Lb102 and Bf141 reduced diet-induced obesity, visceral fat accretion, and inflammation, concomitant with improvement of glucose tolerance and insulin sensitivity. Further analysis revealed that Lb102 and Bf141 enhanced intestinal integrity markers in association with selective changes in gut microbiota composition. We have thus identified 2 new potential probiotic bacterial strains with immunometabolic properties to alleviate obesity development and associated metabolic disturbances.-Le Barz, M., Daniel, N., Varin, T. V., Naimi, S., Demers-Mathieu, V., Pilon, G., Audy, J., Laurin, E., Roy, D., Urdaci, M. C., St-Gelais, D., Fliss, I, Marette, A. In vivo screening of multiple bacterial strains identifies Lactobacillus rhamnosus Lb102 and Bifidobacterium animalis ssp. lactis Bf141 as probiotics that improve metabolic disorders in a mouse model of obesity.

First Authors:
Mélanie Le Barz

Correspondence Authors:
André Marette

All Authors:
Mélanie Le Barz,Noëmie Daniel,Thibault V Varin,Sabrine Naimi,Véronique Demers-Mathieu,Geneviève Pilon,Julie Audy,Émilie Laurin,Denis Roy,Maria C Urdaci,Daniel St-Gelais,Ismaïl Fliss,André Marette