创作:szx 审核:szx 07月11日
  • 纳入56名IBD患者(26名溃疡性结肠炎(UC)、30名克罗恩病(CD))及12名健康人,利用质谱流式细胞技术分析血液或活检组织中的白细胞亚群、功能及肠道归巢分子表达;
  • 血液与活检组织中的白细胞特征存在差异,同时也有一定的相关性;
  • 血液白细胞的特征可反映患者的临床分层,利用血液白细胞的肠道归巢分子表达可区分CD与UC;
  • 鉴定出CD患者与UC患者的16个组织白细胞特征存在显著差异,CD患者的炎症与非炎症组织中白细胞的活化水平不同。
Nature Communications上发表的一项最新研究,利用质谱流式细胞技术,在溃疡性结肠炎患者、克罗恩病患者及健康人之间对比鉴定了血液及活检组织样本中白细胞的特征差异,发现通过检测血液中的免疫细胞特征可区分不同的IBD临床亚型。

Mass cytometry reveals systemic and local immune signatures that distinguish inflammatory bowel diseases



06-19, Article

Abstract & Authors:展开

Inflammatory bowel disease (IBD) includes Crohn's disease and ulcerative colitis. Each disease is characterized by a diverse set of potential manifestations, which determine patients' disease phenotype. Current understanding of phenotype determinants is limited, despite increasing prevalence and healthcare costs. Diagnosis and monitoring of disease requires invasive procedures, such as endoscopy and tissue biopsy. Here we report signatures of heterogeneity between disease diagnoses and phenotypes. Using mass cytometry, we analyze leukocyte subsets, characterize their function(s), and examine gut-homing molecule expression in blood and intestinal tissue from healthy and/or IBD subjects. Some signatures persist in IBD despite remission, and many signatures are highly represented by leukocytes that express gut trafficking molecules. Moreover, distinct systemic and local immune signatures suggest patterns of cell localization in disease. Our findings highlight the importance of gut tropic leukocytes in circulation and reveal that blood-based immune signatures differentiate clinically relevant subsets of IBD.

First Authors:
Samuel J S Rubin

Correspondence Authors:
Aida Habtezion

All Authors:
Samuel J S Rubin,Lawrence Bai,Yeneneh Haileselassie,Gotzone Garay,Chohee Yun,Laren Becker,Sarah E Streett,Sidhartha R Sinha,Aida Habtezion