与HIV感染或高危女性的糖尿病相关的肠道菌群及血浆代谢产物

纳入28名HIV女性感染者(16名患有糖尿病)及20名未感染HIV但感染风险较高的女性(6名患有糖尿病);
糖尿病或HIV感染对肠道菌群的细菌α多样性及β多样性均无显著影响;
相比于无糖尿病的女性,糖尿病女性患者中纤毛菌属等4个细菌属的相对丰度较低,血浆中与色氨酸分解代谢相关的代谢产物水平较高,而甘油磷脂的水平较低;
糖尿病与肠道菌群组成及血浆特定代谢产物的关联不受HIV感染的影响。
延伸阅读
EBioMedicine [IF:6.183]

Gut microbiota and plasma metabolites associated with diabetes in women with, or at high risk for, HIV infection

2018-10-23, Article, 10.1016/j.ebiom.2018.10.037more

Abstract:
BACKGROUND: Gut microbiota alteration has been implicated in HIV infection and metabolic disorders. The relationship between gut microbiota and diabetes has rarely been studied in HIV-infected individuals, who have excess risk of metabolic disorders.
METHODS: Our study during 2015-2016 enrolled predominantly African Americans and Hispanics in the Women's Interagency HIV Study. We studied 28 women with long-standing HIV infection under antiretroviral therapy and 20 HIV-uninfected, but at high risk of infection, women (16 HIV+ and 6 HIV- with diabetes). Fecal samples were analyzed by sequencing prokaryotic16S rRNA gene. Plasma metabolomics profiling was performed by liquid chromatography-tandem mass spectrometry.
FINDINGS: No significant differences in bacterial α- or β-diversity were observed by diabetes or HIV serostatus (all P > .1). Relative abundances of four genera (Finegoldia, Anaerococcus, Sneathia, and Adlercreutzia) were lower in women with diabetes compared to those without diabetes (all P < .01). In women with diabetes, plasma levels of several metabolites in tryptophan catabolism (e,g., kynurenine/tryptophan ratio), branched-chain amino acid and proline metabolism pathways were higher, while glycerophospholipids were lower (all P < .05). Results were generally consistent between HIV-infected and HIV-uninfected women, and no significant modification effects by HIV serostatus were observed (all P > 0.05). Anaerococcus, known to produce butyrate which is involved in anti-inflammation and glucose metabolism, showed an inverse correlation with kynurenine/tryptophan ratio (r = -0.38, P < .01).
INTERPRETATION: Among women with or at high risk for HIV infection, diabetes is associated with gut microbiota and plasma metabolite alteration, including depletion of butyrate-producing bacterial population along with higher tryptophan catabolism. FUND: NHLBI (K01HL129892, R01HL140976) and FMF.

First Authors:
Jee-Young Moon

Correspondence Authors:
Qibin Qi

All Authors:
Jee-Young Moon,Christine P Zolnik,Zheng Wang,Yunping Qiu,Mykhaylo Usyk,Tao Wang,Jorge R Kizer,Alan L Landay,Irwin J Kurland,Kathryn Anastos,Robert C Kaplan,Robert D Burk,Qibin Qi