Nature子刊:包含近800个肠道菌株全基因组数据的新数据集

发布人体胃肠道细菌培养集(HBC)数据,HBC包含了来自4个门、31个科、273个种的737个菌株的全基因组数据;
HBC使已有的人体胃肠道细菌基因组数量增加了37%;
相比于人类微生物组(HMP)计划,包含HBC在内共计1354个基因组的人体胃肠道细菌基因集(HGG)将系统分类效率提高了61%,近50%的序列可被分类到亚种级;
新的HGG提高了对宏基因组样本分类的分辨率,并能鉴定出人肠道菌群中的新菌种,同时增加了41个新描述的功能。
延伸阅读
Nature Biotechnology [IF:35.724]

A human gut bacterial genome and culture collection for improved metagenomic analyses

人类肠道细菌基因组和培养集合用于改进宏基因组分析

02-04, Article, 10.1038/s41587-018-0009-7more

Abstract:
Understanding gut microbiome functions requires cultivated bacteria for experimental validation and reference bacterial genome sequences to interpret metagenome datasets and guide functional analyses. We present the Human Gastrointestinal Bacteria Culture Collection (HBC), a comprehensive set of 737 whole-genome-sequenced bacterial isolates, representing 273 species (105 novel species) from 31 families found in the human gastrointestinal microbiota. The HBC increases the number of bacterial genomes derived from human gastrointestinal microbiota by 37%. The resulting global Human Gastrointestinal Bacteria Genome Collection (HGG) classifies 83% of genera by abundance across 13,490 shotgun-sequenced metagenomic samples, improves taxonomic classification by 61% compared to the Human Microbiome Project (HMP) genome collection and achieves subspecies-level classification for almost 50% of sequences. The improved resource of gastrointestinal bacterial reference sequences circumvents dependence on de novo assembly of metagenomes and enables accurate and cost-effective shotgun metagenomic analyses of human gastrointestinal microbiota.

First Authors:
Samuel Forster,Nitin Kumar

Correspondence Authors:
Samuel Forster,Trevor D Lawley

All Authors:
Samuel Forster,Nitin Kumar,Blessing O Anonye,Alexandre Almeida,Elisa Viciani,Mark D Stares,Matthew Dunn,Tapoka T Mkandawire,Ana Zhu,Yan Shao,Lindsay J Pike,Thomas Louie,Hilary P Browne,Alex L Mitchell,B Anne Neville,Robert D Finn,Trevor D Lawley